OmpA and Bap Genes as Virulence Genes Involved in Biofilm Formation of Acinetobacter baumannii

Background: Multiple virulence genes involved in biofilm development by Acinetobacter Baumannii (A. baumannii) like ompA and bap genes. These genes may be analyzed to establish their relevance in biofilm formation or antibiotic resistance phenotypes. This research objects to study virulence genes involved in biofilm formation of A. baumannii to be taken in consideration in prophylactic approaches, treatment, and infection control program. Methods: The prospective study contained 100 isolates including (sputum, endotracheal aspirate, pus from open wounds or open abscesses, mid-stream urine from non-catheterized subjects and catheter-stream urine in catheterized subjects, blood samples and body fluids) were collected from different ICUs. The BD Phoenix Automated System was used to identify the isolates and assess their susceptibility to 21 antibiotics. Biofilm formation was measured by microtiter plate for all isolates, then conventional PCR was done for detection of ompA and bap genes. Results: The comparison of biofilm strength and MDR revealed significant variance among different groups (P