Application of Exogenous Lipase in the Study of Rice Bran Stabilization

In view of the low content of lipase in rice bran, it is difficult to directly and efficiently measure its lipase activity in the rice bran heat inactivation experiment, an experimental method of adding exogenous lipase was proposed to quickly and accurately detect the lipase activity in the heated rice bran. Taking the correlation between the temperature and time of rice bran heat treatment and the enzyme activity as the starting point, the self-made aluminum heating container with high sealing property was used, and the COMSOL simulation verified that the container met the uniformity requirements of rice bran heating; Take rice bran as the heating medium, add a certain amount of exogenous plant lipase, and then heat the rice bran containing exogenous lipase, and detect the activity of lipase after heating, to explore the application of adding exogenous lipase in rice bran stabilization. The results showed that when exogenous lipase and rice bran were mixed with 1:1 (mass ratio), the lipase activity of the mixture increased in equal proportion; After heat treatment at 110 ℃, 120 ℃ and 130 ℃, the measured change of lipase activity in the mixture was in good agreement with the change trend of rice bran lipase activity, and the difference between different test temperature groups was significant (P