Biological oxygen sensing via two-photon absorption by an Ir(III) complex using a femtosecond fiber laser

Biological oxygen sensing via two-photon absorption by an Ir(III) complex using a femtosecond fiber laser

Near-infrared two-photon absorption of the phosphorescent Ir(III) complex (2,4-pentanedionato-κO2,κO4)bis[2-(6-phenanthridinyl-κN)benzo[b]thien-3-yl-κC]iridium (BTPHSA) was characterized. It exhibited a 800-1200 nm two-photon absorption band, and thus could be electronically excited by 1030-nm femto...

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Veröffentlicht in:Japanese Journal of Applied Physics 2016-09, Vol.55 (9), p.92401
Hauptverfasser: Moritomo, Hiroki, Fujii, Akinari, Suzuki, Yasutaka, Yoshihara, Toshitada, Tobita, Seiji, Kawamata, Jun
Format: Artikel
Sprache:eng
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Zusammenfassung:Near-infrared two-photon absorption of the phosphorescent Ir(III) complex (2,4-pentanedionato-κO2,κO4)bis[2-(6-phenanthridinyl-κN)benzo[b]thien-3-yl-κC]iridium (BTPHSA) was characterized. It exhibited a 800-1200 nm two-photon absorption band, and thus could be electronically excited by 1030-nm femtosecond Ti:sapphire and Yb-doped fiber lasers. By using BTPHSA, oxygen concentrations in human embryonic kidney 293 (HEK293) cells were imaged. These results demonstrate two-photon oxygen sensing of live tissues via easily operable excitation sources.
ISSN:0021-4922
1347-4065
DOI:10.7567/JJAP.55.092401