Imaging of tumor colonization by Escherichia coli using 18 F-FDS PET

Tumor-targeting bacteria have been actively investigated as a new therapeutic tool for solid tumors. However, imaging of tumor-targeting bacteria has not been fully established. F-fluorodeoxysorbitol (FDS) positron emission tomography (PET) is known to be capable of imaging Gram-negative Enterobacteriaceae infection. In the present study, we aimed to validate the use of F-FDS PET for visualization of the colonization and proliferation of tumor-targeting ( ) MG1655 in mouse tumor models. (5 × 10 colony forming unit) were injected intravenously into BALB/c mice bearing mouse colon cancer (CT26). Before and 1, 3, and 5 days after the bacterial injection, PET imaging was performed following i.v. injection of approximately 7.4 MBq of F-FDS. Regions of interest were drawn in the engrafted tumor and normal organs including the heart, liver, lung, brain, muscle, and intestine. Semiquantitative analysis was performed using maximum standardized uptake value (SUV ). F-FDS uptake was significantly higher in tumors colonized by live MG1655 than in uncolonized tumors ( < 0.001). The PET signals in the colonized tumors at 3 days after bacterial injection were 3.1-fold higher than those in the uncolonized tumors. Tumoral F-FDS uptake correlated very strongly with the number of in tumors (r = 0.823, < 0.0001). Cross sectional analysis of autoradiography, bioluminescence, and pathology revealed that the F-FDS uptake sites in tumors matched the locations of MG1655. In conclusion, F-FDS PET is expected to be useful for the semiquantitative visualization of tumor-targeting bacteria when bacterial cancer therapy is performed using Gram-negative Enterobacteriaceae such as .