Investigation of the Purification/Concentration Method for Dioxins Analysis by GC-MS and Bioassay

Our previous report showed that the heating (60°C) multilayer silica gel columns, i.e., AgNO3silica gel and H2SO4silica gel, was useful as purification method for dioxins analysis. In this study, further simplified-and automated-preparation method for dioxins analysis using HRGC-HRMS and Bioassay was studied on three successive steps of the purification, concentration and solvent substitution. The results showed the best suitable condition as follow: An alumina column as concentration and solvent substitution was set under multilayer silica gel column. Dioxins sample in hexane was applied on a top of multilayer silica gel column. After heating the column at 60°C, dioxins were eluted with 60 mlofn-Hexane kept at 60°C, and then followed by drying the alumina column with N2gas. Thereafter, dioxins in alumina column were reversibly eluted with 0.5 mlof Toluene or DMSO solution kept at 60°C, and determined by GC-MS or Bioassay. This newly developed method for dioxins analysis was considered to be useful for reduction of analytical time, amount of solvent, and cost.