Study on the Extraction Method for Dioxin and Related Compounds in Biological Samples

A new extraction method, so named “High Alkali PG method”, was studied in order to develop a simply extraction method of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDDs/DFs) and coplanar polychlorinated biphenyls (Co-PCBs) in biological samples such as whale blubber, cow fat, pork meat, cow liver, chicken meat and fish meat. “High Alkali PG method” was composed of three reagents, water 25 ml-3 %-pyrogallol/ethanol 75 ml-15M-KOH/water 50 ml, and showed high decomposition of protein and fat, high extraction efficiency (73-116 %) of PCDDs/DFs/Co-PCBs, and high reproducibility (CV: < 10 %) of PCDDs/DFs/Co-PCBs. These results indicate that “High Alkali PG method” is useful for analysis of PCDDs/DFs and Co-PCBs in various biological samples.