Investigating the effect of subjecting the Zinacef (750mg) antibiotic to water, tea, milk, grapefruit juice, coffee, and energy drink on its effectiveness in inhibiting growth of Lactobacillus Rhamnosus

Antibiotics are the medications used for treatment of bacterial infections or inhibiting their spread, most frequently by interfering with bacteria metabolism. For instance, the Zinacef antibiotic used in the investigation is a cefuroxime, which is a substance that belongs to cephalosporins antibiotics. Peptidoglycan is responsible for providing structural stability to the cell wall in bacteria. In gram-positive bacteria, such as Lactobacillus Rhamnosus, peptidoglycan is a vital component with a thickness of more than 10 layers, without which the cell cannot function properly. β-lactams antibiotics have been proven to inhibit the last step of peptidoglycan synthesis, thereby depriving the cell of the main protective layer.. However, as mentioned in Fundament of interest, there is evidence that some substances can decrease the efficiency of antibiotics in fighting bacteria. Moreover, some of them have both antimicrobial properties and properties that inhibit the effectiveness of antibiotics at the same time. In this work, an attempt was made to check What is the effect of water, tea, milk, grapefruit juice, coffee, and energy drink on the effectiveness of Zinacef antibiotic in inhibiting growth of Lactobacillus Rhamnosus, investigated by measuring the zones of inhibition with use of a vernier calliper? The results and conclusions of the experiment were as follows: water does not contain substances that could somehow affect the effectiveness of the Zinacef antibiotic, tea properties have the smallest effect on Zinacef antibiotic effectiveness in inhibiting Lactobacillus Rhamnosus growth, in the experiment milk indeed negatively influenced the activity of Zinacef antibiotic. On the other hand energy drinks have blocked the activity of the antibiotic to a greater extent than coffee.