Neutral α-mannosidase from fungus Aspergillus fischeri (NCIM 508) belongs to Class IIC subfamily

BACKGROUD & OBJECTIVE: The α-mannosidase gene from Aspergillus fischeri was isolated by DNA and RNAmethod. METHODOLOGY: Cloning of gene, isolation of recombinant plasmid and sequencing of gDNA gene wascarried out and bioinformatic analysis was done for gene to get intron-exon information and for sequence homology.RESULTS:The α-mannosidase gene from Aspergillus fischeri is 1990 base pair long and sequence analysis showedpresence of three introns and four exons in the gene. BLAST analysis revealed 100% similarity to the Neosartoryafischeri (A. fischeri) and 94% with Aspergillus fumigatus class I α-mannosidase reported in NCBI Gene Data Bank.CONCLUSION: The neutral α-mannosidase from Aspergillus fischeri could belong to the Class IIC subfamily.