A Study on Direct Injection of Mitomycin C, Adsorbed on Small Activated Carbon Particles into the Metastatic Lymph Node in Mice

MH134 cells were inoculated into the footpad of the left hind leg of C3H mice on Day 0, then lymph node metastases were induced at the left popliteal lymph nodes and the lumbar lymph nodes. Mitomycin C (MMC), 25 μg/mouse, was injected into the left popliteal lymph node in the form of MMC-CH (MMC adsorbed on activated carbon particles) or MMC aqueous solution on Day 9. One hour later the MMC activity in the lumbar lymph node was markedly grreater in the MMC-CH group than in the MMC aqueous solution group. Both the weight and the histologically detected metastatic area of the lumbar lymph nodes, that were extirpated on Day 13, were significantly smaller in the MMC-CH group than in the other groups. These results indicate that intralymphnodal injection of MMC adsorbed on activated carbon particles is effective against the distant lymph node metastasis.