Breeding soundness examination in a clouded leopard

A 3-year male clouded leopard was presented to the University of Tennessee College of Veterinary Medicine Theriogenology servicesfor a breeding soundness examination. On physical examination, the left testis felt abnormally small. On ultrasonography,no abnormalities were observed; testis and prostate were of normal size. Semen was collected with a pulsator electroejaculatorand a ram electroejaculator probe (commonly used to collect semen in small ruminants). The purpose of using this electroejaculatorand probe was to determine if they could be used to collect semen from a clouded leopard. Using manual settings with thisdevice we collected ~ 0.2 - 0.3 ml of semen. Motility and morphology of sperm were acceptable. We concluded that the PulsatorIV electroejaculator and ram probe can be used to collect semen as part of breeding soundness evaluation in a clouded leopard.