Experimental and Clinical Studies on Washing Solution used in Lavage Cytology under Direct Vision by Fiberga stroscope

Experimental and clinical studies were carried out to investigate the optimum conditions of the washing solutions in lavage cytology under direct vision by fibergastroscope for the diagnosis of gastric neoplasms. 1) The effects of various washing solutions on preservation of morphology and function of gastric cells were examined experimentally by using cytological, electronmicroscopic and cytochemical methods on exfoliated gastric cancer cells. Hanks' solution was the best among 5 kinds of washing solutions. 2) Effects of various washing solutions on viability and morphological changes of HeLa Cells were investigated. Hanks' solution at an optimum pH 7.4, gave the most excellent results. Effects of osmotic pressure were not significant. 3) When pH of washing solutions was less than 6.0, marked degeneration of cells was observed. Phosphate buffer was more effective in buffer action, but degeneration of cells was more remarkable in phosphate buffer than in Hanks' solution. Excellent results were obtained by adjusting the pH of Hanks' solution to 8.0, when pH of gastric juice was less than 4.0.