Structures of Nuclear Chromatins in Interphase and Alterations of Chromosomes in Ca-in situ and Invasive Carcinoma of the Cervix

A relationship between changes in chromatin structure and chromosomal abnormalities has become of interest. Whereas detailed observation of nuclear chromatin in interphase was difficult in hematoxylin stained specimens by optical microscope and, for this reason, has been variously described as reticular or coagulated. The study demonstrates that the chromatin pattern of normal and malignant cervical cells was observed clearly as chromatin grains by the dark filled, oblique illumination method using polarized monochromatic light. Chromosomes were determined from biopsy by a direct squash technique in15patients with intrae pithelial carcinoma and 42 patients with invasive carcinoma of the cervix. Results reveal the following: 1) The chromatin pattern of a normal cell is observed as a mass of grains which have various sizes and forms by this method.The grain numbers between 40 and 50 are most frequent. 2) On the other hand, chromatin pattern of the malignant cell is observed as a tight mass of grains which have definite size and form, a pattern which seems to be similar to the undifferentiated cells. 3) The grain numbers of the malignant cells in creased widely from 60 to 100 in most cases. 4) In metaphase of malignant cell populations, the chromosome number range was found in two patterns; the first pattern is a clear hyperdiploid mode and other pattern, in addition, is a wide chromosomal range from 40 to excess of 100 which is not a true mode.