Spatio-temporal gene expression analysis from cryosection using laser microdissection

Laser microdissection (LMD), also called as Laser capture microdissection (LCM), is a method for isolating different tissue cells or specific single cells from wide variety of tissue samples under direct microscopic observation. LMD method enables to harvest the cells of interest region or specific cells for several analysis such as DNA/RNA analysis, proteomics, metabolomics and other molecular analysis. Currently, this approach can be used to study various biological events at tissue- or cellular-level, LMD has been used in the wide field of research. In this report, we describe techniques for isolation different tissues/specific cells from cryosections of Arabidopsis incised flowering stem by LMD for spatio-temporal gene expression analyses.