Development and Validation of Novel Gas Chromatography (GC) and Thin Layer Chromatography (TLC) Densitometry Methods for the Quantification of Stigmasterol 3-O-β-D-Glucopyranoside (S3G) in Balanites Aegyptiaca Extract: Application to Newly Formulated Balanites Capsules

Balanites aegyptiaca is a commonly used antihyperglycemic in Egyptian folk medicine. The objective of the present study is to develop two chromatographic methods for standardizing the Balanites extract by quantification of its main component, stigmasterol 3-O-β-D-glucopyranoside (S3G). The first method is gas chromatography (GC) where nitrogen was adopted as the carrier gas. The second method was thin layer chromatography (TLC) densitometry where chromatographic separation was established on aluminum plates using chloroform; methanol was used as the mobile phase followed by a densitometric measurement at 254 nm. Validation of the proposed methods was applied per International Council for Harmonization (ICH) guidelines. Both methods were adopted for quantification of S3G in Balanites capsules. The standard calibration curve was established for S3G in the concentration range of 10–130 μg/mL and 0.5–7.5 μg/mL for the GC and TLC methods, respectively. A good linearity with a correlation coefficient of 0.999 was obtained for both methods. Furthermore, the mean percentage recovery was found to be 99.46% for GC and 100.67% for TLC. Good precision was achieved, with relative standard deviation values