Behavior of Alkaline Lipase on Detergency

The effects of Aspergillus oryzae lipase on the removal of triolein from fabric were determined from the results of the hydrolysis of triolein. Aspergillus oryzae lipase was stable in alkaline solutions and in anionic surfactant solutions and particularly so in an α-olefinsulfonate (AOS) solution. The removal of triolein from fabric was significantly improved by the addition of Aspergillus oryzae lipase. Aspergillus oryzae lipase hydrolyzed triolein into oleic acid without the accumulation of diolein or monoolein. Results of SEM, EPMA and BSE measurement showed the presence of triolein on the lumen of single cotton fibers. The triolein in these fibers can be removed by Aspergillus oryzae lipase.