Lipids of Cancerous Tissue

By ingestion of food with heated lipids, not adenocarcinoma but abnormal growth like tumors is produced in gastric mucous membrane of rats. In general, carcinogenic agents have a close connection with protein and combine with it. Lipids having oxidized unsaturated fatty acids and protein form complexes. Because of their strong linkages, the complexes are not decomposed by solvent extraction. Would the oxidized lipids form complexes with nucleoproteins, the status of cell-division may probablly change. It is thus assumed that the cancerous cells may be produced. In such a case, cancerous tissues may contain oxidized lipids. From this point of view, lipids were extracted from cancerous tissues, such as gastric scirrhous carcinoma, gastric adenocarcinoma, rectal adenocarcinoma and mammary carcinoma indicated the picture of carcinoma simplex tissues. Then fatty acids and their methyl esters were obtained. All procedures were carried out at room temperature under the atmosphere of nitrogen without exposing samples to direct sunlight. From ultra-violet spectrum, fatty acids composition was calculated. It was ascertained that trans-trans conjugated diene hydroperoxide exists in the methyl esters obtained from cancerous tissues, and that trans-trans nonconjugated dienoic acid also exists in some such esters from the results of infrared spectrum analysis.