Measurement of Miconazole Serum Concentration in High-performance Liquid Chromatography

Miconazole (MCZ) serum concentration was measured by high-performance liquid chromatography (HPLC). We assessed whether the internal standard method produced on intra-assay error, and found that the method gave more precise and more reproducible results than absolute calibration curve method. With 0.5μg/ml of MCZ, the coefficient of variation produced by that method was 3.41%, whereas that of the absolute calibration curve method was 5.20%. The concentrations of absolute calibration curve method showed higher values than the internal standard method. This indicated that the internal standard method was far more precise in measuring the MCZ serum concentrations than was the absolute calibration curve method.