Quality Test of Kalliginogenase Preparations

Extractability from capsule or tablet preparations, and quality test were examined on 5 commercial kalliginogenase products. In extraction from tablet or capsule preparations, we ground them to powder with a small amount of water in a mortar. By this simple method, kalliginogenase could be extracted from each preparation. The amounts of protein determined by Lowry method were 10 to 100 times more than that of the standard (1μ/ U), but did not correspond to the esteolytic activity measured by BAEE method. Furthermore, one of them was contaminated with trypsin-like protein in the preparation because its esteolytic activity was repressed by a soybean trypsin inhibitor or urinastatin. Therefore, it seems that highly purified kalliginogenase was not used for all commercial products.