Application of loop-mediated isothermal amplification (LAMP) in the detection of genetically modified RT 73 rapeseed: a qualitative fast inspection

The study intended to establish a rapid, simple, efficient and sensitive method for detecting transgenic RT 73 rapeseed using the loop-mediated isothermal amplification (LAMP) technology. The transgenic RT 73 rapeseed sequences locating at the interface of endogenous gene to exogenous gene was taken as the target sequences, and four LAMP primers, inner primers FIP, BIP, and outer primers F3, B3 were designed, with specific identification to six independent regions of target genes. The LAMP reaction was performed. The LAMP primers specifically amplified the exogenous DNA of transgenic RT 73 rapeseed and the detection limit was 0.01ng DNA.The results could be determined with the help of real-time turbidimeter or directly observed by eyes for the qualitative determination. LAMP was specific to amplify exogenous genes of genetically modified RT 73 rapeseed, and the detection limit of 0.1 ng DNA was similar to that of real-time PCR. The LAMP operation was simple to greatly improve the efficiency of transgenic detection and have a good application prospect.