Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building [Short communication]
The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-process...
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| Veröffentlicht in: | The international journal of tuberculosis and lung disease 2012-11, Vol.16 (11), p.1535-1537 |
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| container_title | The international journal of tuberculosis and lung disease |
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| creator | David, S. Sutre, A. F. Sanca, A. Mané, A. Henriques, V. Portugal, C. Sancho, L. Cardoso, A. Paixão, E. Duarte, E. L. Leite, C. Q. F. Salem, J. I. Antunes, A. |
| description | The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed
and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on
the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings. |
| doi_str_mv | 10.5588/ijtld.11.0658 |
| format | Article |
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and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on
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| ispartof | The international journal of tuberculosis and lung disease, 2012-11, Vol.16 (11), p.1535-1537 |
| issn | 1027-3719 1815-7920 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Antitubercular Agents - pharmacology Bacterial diseases Bacterial Proteins - genetics Base Sequence Biological and medical sciences Capacity Building - methods Clinical Laboratory Techniques Denaturing High-Performance Liquid Chromatography (dhplc) Sequencing DNA-Directed RNA Polymerases Guinea-Bissau Human bacterial diseases Humans Infectious diseases Laboratories - organization & administration Medical sciences Microbial Sensitivity Tests Mycobacterium Tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - isolation & purification Pneumology Portugal Rifampin - pharmacology Rpob Sequence Analysis Sodium Hypochlorite Sodium Hypochlorite - chemistry Specimen Handling - methods Sputum - microbiology Sputum Smear Microscopy Tuberculosis - diagnosis Tuberculosis - drug therapy Tuberculosis - microbiology Tuberculosis and atypical mycobacterial infections |
| title | Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building [Short communication] |
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