Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building [Short communication]
The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-process...
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Veröffentlicht in: | The international journal of tuberculosis and lung disease 2012-11, Vol.16 (11), p.1535-1537 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed
and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on
the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings. |
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ISSN: | 1027-3719 1815-7920 |
DOI: | 10.5588/ijtld.11.0658 |