Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building [Short communication]

The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-process...

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Veröffentlicht in:The international journal of tuberculosis and lung disease 2012-11, Vol.16 (11), p.1535-1537
Hauptverfasser: David, S., Sutre, A. F., Sanca, A., Mané, A., Henriques, V., Portugal, C., Sancho, L., Cardoso, A., Paixão, E., Duarte, E. L., Leite, C. Q. F., Salem, J. I., Antunes, A.
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Sprache:eng
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Zusammenfassung:The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
ISSN:1027-3719
1815-7920
DOI:10.5588/ijtld.11.0658