Suitability of undenatured ethanol for DNA and RNA preservation in pig oral fluid and fecal samples used for PCR-based pathogen detection

Suitability of undenatured ethanol for DNA and RNA preservation in pig oral fluid and fecal samples used for PCR-based pathogen detection

Nucleic acid integrity in pig oral fluid and fecal samples is important for polymerase chain reaction-based pathogen detection and appropriate preservation during shipping is required. A final concentration of 70% undenatured ethanol was sufficient to maintain DNA and RNA quality for up to 7 days.

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Veröffentlicht in:Journal of swine health and production 2024-09, Vol.32 (5), p.213-219
Hauptverfasser: Gerszon, Joanna, Genz, Berit, Moser, Raif, Pollock, Yvette, Sellars, Melony
Format: Artikel
Sprache:eng
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Zusammenfassung:Nucleic acid integrity in pig oral fluid and fecal samples is important for polymerase chain reaction-based pathogen detection and appropriate preservation during shipping is required. A final concentration of 70% undenatured ethanol was sufficient to maintain DNA and RNA quality for up to 7 days.
ISSN:1537-209X
DOI:10.54846/jshap/1383