Rabies virus in McCoy cell line. Part 1 - Cytopathic effect and replication

The cytopathic effect caused by rabies virus is not easy to detect, although the McCoy cell line showed high sensitivity to this virus. The titre increased at each new passage and the ERA strain reached titre higher than 10 8.0 LD 50/ 0,03 ml after eight successive passages only. The kinetics of infection showed that 48 hours after inoculation, the cell reaches the peak of infecton and the celular proteins synthesis is inhibited. The data are reproducible suggesting that this cell line could be used for diagnostic purposes and for virus mass production.