Normative data for macular volume with high-definition spectral-domain optical coherence tomography (Spectralis)

To establish normative data for macular volume by spectral-domain optical coherence tomography (SD-OCT) in subjects with no known retinal disease. In an academic institutional setting, 50 subjects (age range 20-84 years) with no known retinal disease, best-corrected visual acuity 20/20, and normal intraocular pressure were enrolled. The subjects were divided into 3 age groups: group 1 included subjects 20-40 years of age, group 2 included subjects 41-60 years of age, and group 3 included subjects 61 years of age and older. All subjects underwent a complete ophthalmologic examination and testing, if needed, to rule out any retinal diseases or glaucoma. Retinal volume (RV) in 9 Early Treatment Diabetic Retinopathy Study (ETDRS) subfields and total macular volume (TMV) inclusive of all 9 subfields were analyzed. Statistical analyses were carried out using analysis of variance. Overall, the mean TMV ± SD for ETDRS circles with a diameter of 1, 2.22, and 3.45 mm was determined to be 3.04 ± 0.14 mm3. Among the ETDRS subfields, RV was highest in the nasal outer field, with a mean of 0.47 ± 0.02 mm3. The TMV did not show a significant difference with age (p = 0.17). However, TMV was significantly higher in males (p = 0.003) and in Caucasian and Asian subjects compared to African Americans (p = 0.017). Normative values for total macular volume in ETDRS subfields in otherwise healthy eyes were measured to be 3.04 ± 0.14 mm3.