Analysis of Glucosamine in Cream Dosage Form and Diffusion Liquid by High Performance Liquid Chromatography

A simple and sensitive high performance liquid chromatography (HPLC) method based on a reaction of 1-naphthyl isothiocyanate (NITC) with glucosamine was developed and validated to determine glucosamine in cream dosage form and diffusion liquid. The derivatisation products were cleaned up using styrene divinylbenzene quaternary ammonium ion exchanger and was then analyzed on a reverse-phase C18 column (150 mm × 4.6 mm, 5 μm) using acetonitrile-water-triethylamine (15: 85: 0.05) as the mobile phase and was detected at wavelength of 254 nm. The detector response was linear in the concentration range of 1-1200 μg/ml. The recovery of glucosamine from cream and diffusion liquid were 95.9-97.4% and 97.6-98.4%, respectively. The intra- and inter-day relative standard deviation (%RSD) was less than 5%.