Bacterial Isolates and their Antimicrobial Susceptibility Pattern from Tracheal Secretions and Sputum of Admitted and OutdoorPatients

Objective: The purpose of study was to determine the frequent pathogens in tracheal secretions and sputum specimens along with the antimicrobial susceptibility pattern. Methods: This descriptive study was conducted in the department of Pathology and Microbiology, Farooq Hospital Lahore, Pakistan and data included was taken from 1st August 2022 to 31st December 2022. The specimens were inoculated on blood agar and MacConkey agar and incubated for 24 hours at 37 0 C. The Kirby Bauer disc diffusion method was employed to find out the antimicrobial susceptibility pattern. The SPSS was used to assess the data. Results: From the total 102 positive culture growths, Klebsiella species (35.2%) was frequently isolated followed by Pseudomonas species (25.4%), Acinetobacter species (23.5%), Escherichia coli (9.8%), Staphylococcus aureus(3.9%), Citrobacter species (0.9%), and Proteus species (0.9%). The doxycycline and linezolid were completely effective (100%) against the gram positive cocci. The Klebsiella species, Pseudomonas species, Acinetobacter species, and Escherichia coli, showed maximum sensitivity to tigecycline. Conclusion: The most commonly isolated gram negative rod was Klebsiella species and tigecycline was found to be the most effective antibiotic against it. Multidrug resistance among respiratory pathogens is the major issue so it is necessary to administer antibiotic therapy in a limited and objective manner