Isolation and molecular identification of the invA gene of Salmonella spp. in dromedary camels

This study was done to determine the percentage of Salmonella spp. in camels from three provinces (Karbala, Al-Najaf and AL-Muthana) in Iraq with different age and both sexes. Total of 250 fecal samples from 250 camels were collected. Diagnostic study depended upon the morphological and cultural properties of the isolates on some selective media like Xylose lysine deoxycholate (XLD) and Salmonella Shigella (SS) agars which were used in addition to different biochemical tests and molecular assay by PCR for detection of virulence gene invasion A (invA) with Phylogenetic study. The clinical signs appearing on animals infected with Salmonella were greenish diarrhea, loss of appetite with mild systemic reaction. Bacteriological and molecular tests revealed isolation of five Salmonella isolates with invA gene. Two of these isolates were sequenced. The results showed that the first strain S. enterica subspecies typhimurium (LC730846) converged with a group of global strains with one node, as it converged with the global strain that held the clade (MK017934.1 and MT460418.1). While the second local strain S. enterica serovar enteritidis (LC730849) appeared with a new node and it is not affiliated with any association with the world S. enterica strains. It is concluded that the presence of Salmonella spp. in camel needs monitoring in order to minimize the risks of infection exposed the human beings. This study was done to determine the percentage of Salmonella spp. in camels from three provinces (Karbala, Al-Najaf and AL-Muthana) in Iraq with different age and both sexes. Total of 250 fecal samples from 250 camels were collected. Diagnostic study depended upon the morphological and cultural properties of the isolates on some selective media like Xylose lysine deoxycholate (XLD) and Salmonella Shigella (SS) agars which were used in addition to different biochemical tests and molecular assay by PCR for detection of virulence gene invasion A (invA) with Phylogenetic study. The clinical signs appearing on animals infected with Salmonella were greenish diarrhea, loss of appetite with mild systemic reaction. Bacteriological and molecular tests revealed isolation of five Salmonella isolates with invA gene. Two of these isolates were sequenced. The results showed that the first strain S. enterica subspecies typhimurium (LC730846) converged with a group of global strains with one node, as it converged with the global strain that held the clade (MK017934.1 and MT460418.1). W