Utilization of Trametes versicolor for the production of laccase and its application in oxytetracycline degradation from wastewater

The performance of Trametes versicolor following its growth in different lignocellulosic substrates was evaluated. Results showed that the maximum laccase activity was observed in the OPEFB medium, 2051.189 U/L, which is 3.23 folds higher than basal mediums like malt and glucose. The optimum concentration of OPEFB and pH for the submerged culture of T. versicolor were obtained at 30 g/L and pH 5.5, respectively. OPEFB can be fully utilized as a medium for the growth of T. versicolor thus, the wasteland can be minimized. The performance of T. versicolor in basal medium and OPEFB was evaluated using antibiotic oxytetracycline as model emerging micropollutant that contained in wastewater. It was observed that OTC degradation performance (percentage degra­ dation %) of the T. versicolor in OPEFB medium with mycelium from T. versicolor culture degraded almost 99% within 50 h at T48 compared to basal medium with T. versicolor’s mycelium. These results demonstrated that mycelium T. versicolor could offer better OTC degradation performance since the mycelia of T. versicolor absorbed the OTC and then underwent a degradation process.