Influence of coagulant Moringa oleifera seed extract on reduction of Toxoplasma gondii oocysts and viability of Toxocara spp. eggs in water

This work provides insights of coagulant Moringa oleifera (MO) and aluminum sulfate (AS) on reduction of Toxoplasma gondii oocysts and viability of Toxocara spp. eggs in water. Raw (RW) and distilled water (DW) samples were added with 106 oocysts and 800 eggs L–1, before coagulation/flocculation/sedimentation process, the samples were analyzed for oocysts/eggs removal, and eggs viability. The qPCR demonstrated that MO reduced 87.9% and AS, 97.8% of oocysts. Due to their high density, the reduction of eggs was 99%–100%. The eggs viability demonstrates that MO was able to reduce the infective eggs and increase the number of free larvae (non-infecting). In electronic microscopy, a thinner MO layer was observed on the eggs. MO was able to reduce 72.2% of turbidity in RW, while AS reduced 95.8%. MO reduced the quantity of T. gondii oocysts and viability of Toxocara eggs, consequently, the transmission, being useful, low cost, and biodegradable coagulant.