Effect of diode pumping solid state laser with wavelength 589 nm on gene expression of interlukine-2 and interferon-gamma Lowercase in human T-lymphocytes

Objective: To evaluate the impact of laser irradiation of human T-cell lymphocyte in culture to determine thephotobiomodulatory effects of such irradiation on gene expression, and the release of growth factors and cytokines.Method: The experimental study was conducted from November 2021 to April 202...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of the Pakistan Medical Association 2024-09, Vol.74 (10 (Supple-08)), p.S352-S355
Hauptverfasser: Marwa Akram Nafe'a, Nibras Hatim Khamees, Mustafa Salih Ali Almusawi
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Objective: To evaluate the impact of laser irradiation of human T-cell lymphocyte in culture to determine thephotobiomodulatory effects of such irradiation on gene expression, and the release of growth factors and cytokines.Method: The experimental study was conducted from November 2021 to April 2022 at the Postgraduate Anatomy Laboratory, Mustansiriyah University, Baghdad, Iraq, and comprised human blood samples T-cell lymphocytes were extracted and irradiated, followed by the extraction of ribonucleic acid to assess the effect of laser on gene expression of cytokines, interlukine-2 and interferon-gamma. A 589nm yellow light low-level laser beam from a diode pumping solid state laser was used at doses 30J/cm2, 50J/cm2 and 70J/cm2 for an exposure time of 15 minutes. Reverse transcriptase-polymerase chain reaction was used to measure gene expression. Data was analysed using GraphPad Prism 9.Result: Cellular gene expression of the mediators changed significantly in response to laser irradiation at a variety of dosage parameters, and these effects depended on the wavelength and radiation exposure (p
ISSN:0030-9982
0030-9982
DOI:10.47391/JPMA-BAGH-16-80