Effect of a hot water-soluble extraction from Grifola frondosa on the viability of a human monocyte cell line exposed to mitomycin C

The effects of a hot water-soluble extract of Grifola frondosa on the viability of multiple immune cell lines were examined in vitro. The extract and the 1000-cut extract, a low molecular weight fraction, improved the viability of a human monocyte cell line, THP-1, treated by the anticancer drug mitomycin C. DNA microarray and immunoassay results revealed increases in interleukin (IL)-8 expression by THP-1 cells in response to the 1000-cut extract. These data suggest that the extract would enhance the immune system through induction of IL-8 production by human blood monocytes, which leads to activated neutrophils.