A Simple and Rapid Method for the Determination of 5'-Ribonucleotides by Cation Exchanger

A Simple and Rapid Method for the Determination of 5'-Ribonucleotides by Cation Exchanger

Cation exchange chromatographic method proposed by S. Katz and D.G. Comb was modified for determination of 5'-ribonucleotides. By using Dowcx-50 (H+) column, 1.0×13.0cm2, authentic 5'-ribonucleotides, AMP, GMP, IMP, ATP, and acid-soluble ribonucleotides in the muscles of both carp and scal...

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Veröffentlicht in:Eiyo To Shokuryo 1964, Vol.17(4), pp.278-282
Hauptverfasser: Arai, Ken-ichi, Tanaka, Tsune, Saito, Tsuneyuki
Format: Artikel
Sprache:jpn
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Zusammenfassung:Cation exchange chromatographic method proposed by S. Katz and D.G. Comb was modified for determination of 5'-ribonucleotides. By using Dowcx-50 (H+) column, 1.0×13.0cm2, authentic 5'-ribonucleotides, AMP, GMP, IMP, ATP, and acid-soluble ribonucleotides in the muscles of both carp and scallop were separated quantitatively and determined. The method was applied successfully to the determination of the quantities as small as 100μg of individual 5'-ribonucleotides.
ISSN:1883-8863
1883-8863
DOI:10.4327/jsnfs1949.17.278