Particle Size Analysis for Micro-Particles by Capillary Hydrodynamic Chromatography

Particle size analysis for micro-particles by capillary hydrodynamic chromatography was studied experimentally. Retention times of particles depended on their diameter but were independent of their concentration.The linearity of the peak area with particle concentration was found. It is shown that the particle diameter and its concentration can easily and rapidly be analyzed by capillary hydrodynamic chromatography. Resolution of peaks were calculated from chromatograms of binary-particle systems. Particle diameters of a binary-particle system for complete peak separation were predicted from the resolution of the peaks. The mechanism of capillary hydrodynamic chromatography was discussed by considering the dependence of the flow rate of solvent on the retention time, the resolution of the peak and the peak width.