Simultaneous determination of levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma by an HPLC-MS/MS method

Simultaneous determination of levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma by an HPLC-MS/MS method

In this study, we developed and validated a HPLC-MS/MS method capable of simultaneously determining levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma. RESULTS & METHODOLOGY: Chromatographic separation was achieved using a C8 column with a mobile phase consis...

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Veröffentlicht in:Bioanalysis 2015, Vol.7 (2), p.207-220
Hauptverfasser: Ribeiro, Rômulo Pereira, Gasparetto, João Cleverson, de Oliveira Vilhena, Raquel, Guimarães de Francisco, Thais Martins, de Francisco, Thais Martins Guimarães, Martins, Cleverson Antônio Ferreira, Cardoso, Marco André, Pontarolo, Roberto, de Carvalho, Katherine Athayde Teixeira
Format: Artikel
Sprache:eng
Schlagworte:
Benzophenones - blood
Benzophenones - standards
Blood Chemical Analysis - methods
Carbidopa - blood
Carbidopa - standards
Catechols - blood
Catechols - standards
Chromatography, High Pressure Liquid - standards
Dihydroxyphenylalanine - analogs & derivatives
Dihydroxyphenylalanine - blood
Dihydroxyphenylalanine - standards
Dopamine - blood
Dopamine - standards
Humans
Levodopa - blood
Levodopa - standards
Nitriles - blood
Nitriles - standards
Nitrophenols - blood
Nitrophenols - standards
Quality Control
Reproducibility of Results
Tandem Mass Spectrometry - standards
Tyrosine - analogs & derivatives
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Beschreibung
Zusammenfassung:In this study, we developed and validated a HPLC-MS/MS method capable of simultaneously determining levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma. RESULTS & METHODOLOGY: Chromatographic separation was achieved using a C8 column with a mobile phase consisting of a gradient of water and acetonitrile:methanol (90:10 v/v), both containing 0.1% formic acid. The developed method was selective, sensitive (LD0.99), precise (RSD
ISSN:1757-6180
1757-6199
DOI:10.4155/bio.14.230