Screening and Toxicity Assay of Bioactive Compounds from Ethyl Acetate Extract of Indonesian Marine Sponge Dysidia sp. from Hoga Island

Screening of the toxic active compounds from the ethyl acetate fraction of the sponge Dysidea sp. from Hoga Island, Wakatobi National Park. This study aims to determine the toxic properties of the ethyl acetate fraction of sponge Dysidea sp. Isolation was carried out by extraction method using methanol as solvent, then the extract was partitioned using various solvents, such as N-Hexane, Dichloromethane, Ethyl Acetate and Acetone. The obtained fraction was then tested for toxicity using the Brine Shrimp Lethality Test (BSLT) and phytochemical tests. Identification of compounds from the fraction that have toxic properties were then identified using Liquid Chromatography – High Resolution Mass Spectrometry (LC-HRMS). The ethyl acetate fraction obtained was 1.5 grams in the form of a green solid paste. LC-HRMS analysis showed that the ethyl acetate fraction contained 3 reported compounds and 2 unreported compounds.