Fucoxanthin Extraction by Ultrasonic-Assisted from Brown Seaweed (Padina Sp) Origin Pulau Merak Banten

Seaweed is an underutilized resource with great potential in the food industry. Such as cosmetics, agrochemicals, biomass ingredients, bioenergy modifies. Indonesia is a country where two-thirds of its territory is marine. Fucoxanthin is a group of carotenoids found in abundance in marine macroalgae and has various health benefits. Traditional extraction (CE) of such potential biomolecules entails multiple steps that are both laborious and time-consuming. This study used ultrasound-assisted extraction (UAE) to extract high percentages of Fucoxanthin and phenolic compounds from the macroalga Padina sp. This research aims are to identification fucoxanthin compound in Padina Sp, determine the total phenolic content and composition of the pigment of Padina sp. This study also evaluates the effect of the extraction method concerning the time of exposure and solvent concentration on total phenolic content. Extract Padina Sp was further purified and characterized using FTIR. The pigment identification process is carried out by the thin layer chromatography method (TLC) and weigh mol using LC-MS. The results of the analyses present the highest content of TPC in Padina (755.633 mg GAE/mg) on 70 0C at 80% solvent concentration. Based on RSM methodology at 70 0C and 90 % solvent concentration. The active fraction of Fucoxanthin was identified at a Rf 0.96. The results of the RSM (Response Surface Methodology) showed the effect of concentration and temperature on the extraction of Fucoxanthin. The molecular weight of Fucoxanthin was found to be 659.43 m/z