Analysis of bacterial growth in dialysate for CAPD (Dianeal™)

Influence of culture medium and glucose concentration on bacterial growth in Dianeal™ was studied. Various media were inoculated with Dianeal™ containing 105, 103 or 101cfu/ml of S. epidermidis, S. aureus, E. coli or P. aeruginosa. The media used included blood-agar, culture bottle and Uricult™. P.aeruginosa did not grow in the culture bottle medium below a concentration of 103cfu/ml During a 24 hour incubation with these organisms in 1.5 or 2.5% Dianeal™, inhibition of bacterial growth was observed in S. epidermidis, S. aureus and P. aeruginosa. This result showed no changes according to glucose concentrations or osmotic values of Dianeal™. This tendency was obvious in cases in which the bacterial inoculum was small. P. aeruginosa showed the most remarkable tendency. However, the growth of E. coli was not inhibited when the inoculum was more than 103cfu/ml Our results demonstrate that bacterial growth can be inhibited under special conditions such as a high osmolar environment. A study using peritoneal dialysis effluent should be condecuted in the near future.