A STUDY ON BACTERICIDAL ACTIVITY OF CITRATED WHOLE BLOOD

Purpose of this experiment was to find a quantitative indication for bactericidal activity of citrated whole blood in which was represented at 37°C. When the experimental materials were removed from the incubator at 37°C to it at 4-6° C, and then cultured, the logarithmic growth curve of surviving bacteria in this materials was parallel to the curve of survival bacteria in bouillon under the same experimental condition. The experiments showed that the logarithmic growth curve of surviving bacteria was regularly constant, and the proportion of the number of bacteria counted in the beginning was almost parallel to the distance of the position between the curves of these bacteria. It was found that the bactericidal activity might be expressed on the number of days (L-Index) from which the distance was calculated by a general arithmetric formula for computing a method of relative potency. The computated standard was found to be a curve of logarithmic surviving bacteria-growth in bouillon. The computated distance was represented by the diference of the positions between the growth curve in materials and in bouillon. The potency of bactericidal activity in test materials could be expressed by the number of L-Index. Studies on the mechanism of bactericidal activity in human stored blood was investigated by this calculating method. The results of this studies pointed out that this method of the expression on bactericidal activity could be used in practice.