PRODUCTION OF MONOCLONAL ANTI-D FOR USE IN TYPING BLOOD GROUPS

A monoclonal anti-D typing reagent was prepared in following ways. The monoclonal anti-D was generated in hybridoma cell line (DBF-11) established by the use of “EBV-Hybridoma Technique”, in which an Epstein-Barr Virus (EBV)-transformed monoclonal anti-D (IgG1, κ chain) producing human B-cell line (B2-1) was fused with non-immunoglobulin producing JMS-3 cell. Mass culture of the cell line (DBF-11) was continued for the period of over 131 days, by using the continuous cell culturing apparatus (Bio-Pro) provided with “vitafiber”. Weight of the monoclonal anti-D obtained from 8.17 liters of the cultured supernatant fluid was 5.1g expressed in terms of human IgG. After being roughly purified with “hollowfiber”, the IgG content was 4.4g. The Fc portion of the roughly purified IgG (40μg/ml) was bound with mouse anti-human IgG (Fc monospecific antibody: 32μg/ml) so that the monoclonal anti-D was rendered suitable for a blood typing reagent.