Simultaneous quantification of the major flavonoids from wild spinach by UHPLC-HRMS and their neuroprotective effects in a model of H2O2-induced oxidative stress on SH-SY5Y cells

A modified UHPLC-HRMS method for simultaneous quantification of eight flavonoids from the aerial parts of the wild spinach ( Chenopodium bonus-henricus L.) was re-validated for specificity, the limit of detection and quantitation limit, linearity, accuracy, and precision. The glycosides of spinacetin ( Chbhnf-04, Chbhnf-06 , and Chbhnf-08 ) and patuletin ( Chbhnf-01 ) were the predominant compounds. The total amount of assayed flavonoids from the aerial parts of a title plant was estimated to be 1.82% and 1.4% in two different populations from Vitosha Mountain (Bulgaria). The neuroprotective properties of compounds Chbhnf-02 , Chbhnf-04 , Chbhnf-06 , Chbhnf-07 , Chbhnf-08 were further assessed using a model of H 2 O 2 -induced oxidative stress on human neuroblastoma SH-SY5Y cells. All tested flavonoids demonstrated statistically significant neuroprotective activity close to that of silibinin. Patuletin ( Chbhnf-07 ) and spinacetin (Chbhnf-08) triglycosides showed the most protective effects at the lowest concentration of 50 µM.