Interactions of Modern Aesthetic Materials for Prosthetic Restorations with Oral Mucosa A pilot study

The specific objective of the present study is to assess the interactions between cells from a human gingival epithelial cell line and various aesthetic materials used in modern prosthetic dentistry. For this study six types of dental materials were selected: Cr-Co non-precious metal alloy, ceramics applied on Cr-Co non-precious metal alloy, zirconia, ceramics applied on zirconia, polymethyl methacrylate and pressed ceramics/lithium disilicate. Cells from a human gingival epithelial cell line, Ca9-22 (Health Science Research Resources Bank), were cultured on the chosen surfaces for 3, 5 and 7 days. Cellular proliferation, cell attachment (using Multiplex Arrays Technology) and cytotoxicity (MTT- Assay) were evaluated at distinct predetermined intervals. Measurements performed at each distinct predetermined interval showed no significant difference for cell proliferation and cytotoxicity between the selected surfaces, however the highest levels were registered for the polymethyl methacrylate surface. Different attachment patterns were observed for epithelial cells attached on substrates, such as significantly different levels of adherence of E-Cadherin and N-Cadherin molecules; E-Cadherin adhesion levels indicate that pressed ceramics may be the dental material which, compared to the selected materials, influences the least the homeostasis of oral mucosa.