Biological Screening of Triherbal Formulation on Chemically Induced Hepatocellular Carcinoma

The hepatoprotective activity of a triherbal formulation (LAB) comprising of 50 % ethanolic extract of Lawsonia innermis, Aeagel marmelos, Boerrhavia diffusa was studied using Swiss albino rats. The animals received a single intraperitoneal injection of N-nitrosodiethylamine 200mg/kg body wt followed by subcutaneous injection of CCl4 in a dose of 3 ml/kg body wt. The administration of Lawsonia innermis, Aeagel marmelos, Boerrhavia diffusa extracts and cisplatin decreased the liver weight and average liver weight, which shows the rehabilitating capability of extracts in respect with anticancer potency in comparison with the very much effective in preventing NDEA-induced multistage hepatocarcinogenesis possibly through antioxidant and antigenotoxic nature, which was confirmed by various liver injury and biochemical tumor markers enzymes and molecular events. LAB dose dependently and significantly the increase in serum hepatic enzyme levels after NDEA& CCl4 treatment compared to the toxin control group. On administration of LAB, it dose dependently resulted in an elevation in the levels of serum CAT, GPX, GSH, GST, SOD and total protein and decrease in lipid peroxidation compared to the toxin control group. It also increased the attenuated the total protein concentrations compared to the toxin control group. The results of this study confirmed the antioxidant and hepatoprotective activity of the triherbal formulation against carbon tetrachloride& N-nitrosodiethylamine induced hepatotoxicity in Swiss albino rats. © 2011 IGJPS. All rights reserved.