Matrix Metallopeptidase 9 Expression in Tumoral and Marginal Tissues of Cholesteatoma

Matrix Metallopeptidase 9 Expression in Tumoral and Marginal Tissues of Cholesteatoma

Background: Cholesteatoma, a serious, expanding otolaryngologic condition, is challenging to otolaryngologists worldwide. The purpose of the present study was to evaluate the expression of MMP-9 gene in cholesteatoma patients. Methods: In this study, 40 samples of tumor tissue and 40 samples as cont...

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Veröffentlicht in:ImmunoAnalysis 2024-09, Vol.4, p.8
Hauptverfasser: Jabbari Moghaddam, Yalda, Naderpour Germi, Masoud, Noei Alamdary, Shabnam, Emami, Milad, Shanehbandi, Dariush, Razi Soofiyani, Saiedeh
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container_start_page 8
container_title ImmunoAnalysis
container_volume 4
creator Jabbari Moghaddam, Yalda
Naderpour Germi, Masoud
Noei Alamdary, Shabnam
Emami, Milad
Shanehbandi, Dariush
Razi Soofiyani, Saiedeh
description Background: Cholesteatoma, a serious, expanding otolaryngologic condition, is challenging to otolaryngologists worldwide. The purpose of the present study was to evaluate the expression of MMP-9 gene in cholesteatoma patients. Methods: In this study, 40 samples of tumor tissue and 40 samples as control of tumor margins of patients with cholesteatoma were examined. Quantitative real-time polymerase chain reaction (PCR) was used for detection of the MMP-9 gene expression levels in cholesteatoma and control group. expression changes were calculated by ΔCT formula and data were analyzed by GraphPad Prism 8. Results: MMP-9 expression level in case group was significantly higher than the control group (P=0.0011). ROC analysis for the MMP-9 gene expression level that discriminates Cholesteatoma tumoral tissue from healthy tissues revealed a sensitivity of 67.5 % and a specificity of 84.62% with an area under the curve (AUC) value of 0.75 (P
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The purpose of the present study was to evaluate the expression of MMP-9 gene in cholesteatoma patients. Methods: In this study, 40 samples of tumor tissue and 40 samples as control of tumor margins of patients with cholesteatoma were examined. Quantitative real-time polymerase chain reaction (PCR) was used for detection of the MMP-9 gene expression levels in cholesteatoma and control group. expression changes were calculated by ΔCT formula and data were analyzed by GraphPad Prism 8. Results: MMP-9 expression level in case group was significantly higher than the control group (P=0.0011). ROC analysis for the MMP-9 gene expression level that discriminates Cholesteatoma tumoral tissue from healthy tissues revealed a sensitivity of 67.5 % and a specificity of 84.62% with an area under the curve (AUC) value of 0.75 (P&lt;0.001). Conclusion: The results indicated that MMP-9 is overexpressed in cholesteatoma tumoral tissues group compared to the healthy tissues. 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title Matrix Metallopeptidase 9 Expression in Tumoral and Marginal Tissues of Cholesteatoma
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