Early type 2 diabetes and obesity does not affect eicosanoids level and renal morphology in a rat model/ Diabetes tipo 2 no estágio inicial e obesidade não afetam o nível de eicosanóides e a morfologia renal em um modelo de rato

This study evaluated the effects of the early development of Diabetes Mellitus 2 (T2D) and diet-induced Obesity in the eicosanoid pathways and its effects on renal tissue. Thirty male Wistar rats were fed with a high-fat or standard diet and were divided into 3 groups: The Control group received a standard diet, the T2D group received a high-fat diet and a single dose of streptozotocin (25mg/Kg) and the Obesity group received high-fat diet. Caloric intake, feed efficiency, body weight gain, visceral fat, blood glucose, plasma levels of 14,15 EET/DHET, 20-HETE, and kidneys’ morphology were analyzed. Total caloric intake and feed efficiency were higher in the animals of the Obesity group than in Control. Body weight gain, visceral fat, and blood glucose were higher in Obesity and T2D induced groups than in Control. Body weight gain, visceral fat, and feed efficiency associated positively with blood glucose. However, there was no difference in 14,15 EET/DHET, 20-HETE levels, or kidney injury between groups. In conclusion, we were unable to assess whether changes in eicosanoids are due to obesity or diabetes induction. So, this study suggests that longer periods of homeostatic disturbance caused by these protocols seem to be necessary to induce complications related to the disruption of the eicosanoid’s pathway and its effects on renal tissue.