Temperature-Dependent Parameters in Enzyme Kinetics: Impacts on Enzyme Denaturation

Enzymes are vital proteins in biological systems, responsible for regulating and coordinating numerous essential processes. The incorporation of denaturation rate accounts for the gradual loss of enzyme activity over time, which is particularly significant under experimental conditions where enzymes are susceptible to denaturation. It is noteworthy that adverse environmental conditions, such as high temperature or pH imbalance, can induce enzyme denaturation, leading to a loss of functionality over time. This structural disruption renders enzymes inactive, posing a crucial consideration in long-term enzyme kinetics studies. Furthermore, enzymes typically exhibit reduced catalytic activity at lower temperatures, which is pivotal for understanding their stability and efficacy in biological systems and industrial applications. Accordingly, we developed a mathematical model to investigate enzyme kinetics under varying temperature, aiming to analyse their respective impacts on both enzyme behaviour and product formation.