In vitro Propagation of Vanda testacea (Lindl.) Reichb.f. – A Rare Orchid of High Medicinal Value

Foliar explants of Vanda testacea (Lindl.) Reichb. f. were cultured on Mitra (M) medium with 1.0 mg/l BAP, Kn  each and 1.0 mg/l NAA individually and in combination for initiation of regeneration response, proliferation of regenerants and subsequent development of plantlets. Juvenility of the tissue...

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Veröffentlicht in:Plant tissue culture & biotechnology 2010-01, Vol.19 (1), p.1-7
Hauptverfasser: Kaur, Saranjeet, Bhutani, K.K.
Format: Artikel
Sprache:eng
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Zusammenfassung:Foliar explants of Vanda testacea (Lindl.) Reichb. f. were cultured on Mitra (M) medium with 1.0 mg/l BAP, Kn  each and 1.0 mg/l NAA individually and in combination for initiation of regeneration response, proliferation of regenerants and subsequent development of plantlets. Juvenility of the tissues and chemical stimulus were important factors in initiating the regeneration response in the explants. The relatively older leaf explants (>1cm in length) remained recalcitrant to regeneration the representing younger ones (
ISSN:1817-3721
1818-8745
DOI:10.3329/ptcb.v19i1.4077