Callus induction and plant regeneration of paederia foetida L., a widely used medicinal vine in Bangladesh

An efficient protocol was established for in vitro plant regeneration of Paederia foetada L. (Family. Rubiaceae), a widely used medicinal vine of Bangladesh through callus culture in using nodal segment. Yellowish green nodular callus was observed from nodal segments on MS basal medium supplemented...

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Veröffentlicht in:Bangladesh journal of scientific and industrial research 2013-03, Vol.47 (4), p.373-378
Hauptverfasser: Hassan, AKMS, Roy, CK, Sultana, R, Khatun, R
Format: Artikel
Sprache:eng
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Zusammenfassung:An efficient protocol was established for in vitro plant regeneration of Paederia foetada L. (Family. Rubiaceae), a widely used medicinal vine of Bangladesh through callus culture in using nodal segment. Yellowish green nodular callus was observed from nodal segments on MS basal medium supplemented with 1.5 mg/L BAP + 0.5 mg/L NAA within three weeks. Large number of shoots (14.4±1.29) were obtained when the callus was sub cultured on MS medium with 0.5 mg/L BAP. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/L IBA. The survival rate of plantlets was found to be 85%. Regenerated plants were morphologically uniform having normal leaf shape and growth. Bangladesh J. Sci. Ind. Res. 47(4), 373-378, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.14066
ISSN:0304-9809
2224-7157
DOI:10.3329/bjsir.v47i4.14066