Isolation, Identification and Antibiotic Susceptibility Pattern of Urinary Tract Infection Bacterial Isolates

Recently, the world's problem has become in the spread of microbes and the extent of their impact on public health, especially the problem of urinary tract infections (UTIs). Therefore, the purpose of the study was to clarify the extent of infection of the urinary tract and the extent of development of these microbes in resistance to antibiotics (antibiotic susceptibility pattern). The pathogens are isolated from hospitals and private medical centers. In this study, two hundred twenty-five urine samples were collected from various sources, where 126 patients were infected with bacteria while 99 patients had no infection. In Gram-negative bacteria, the most common bacteria were E. coli (55.5%) followed by Klebsiella spp (23.0%), Proteus spp (7.14%), Pseudomonas spp (6.34%), and Acinetobacter spp (3.96%). In the Gram-positive bacteria Staphylococcus spp (2.38%) and Enterococcus spp (1.58%) were found. Antibiotic susceptibilities for Gram-negative and positive bacteria were investigated according to clinical laboratory standard institute(CLSI 2019). In Gram-negative bacteria, Amikcin, Impinem, and levofloxacin were sensitive. However, in Gram-positive bacteria, levofloxacin, ofloxacin, and linezolid were sensitive. At the same time, most of the remaining antibiotics are 100% resistant to pathogenic-bacterial isolates. This study showed bacteria that are isolated from urine which causing urinary tract infection and showed resistance to almost all antibiotics, so UTI is difficult to treat by common antibiotics.