A study on micro flow visualization using evanescent light source

This study aims at visualization of the flow near the wall surface in microchannelusing evanescent wave on a near-field optical microscope. A system was developed for the visualization, which consists of total reflection fluorescent microscopy, Intensified CCD camera, PIV image processing system, microchannel, syringe pump. Including Brownian motion of particle with diameter of 100nm or less was evaluated. Flowanalysis near the surface of a wall was performed. The influential factors onvisualization of micro-flow were evaluated quantitatively. The flow rate near the surfacein a microchannel is measured. The validity of developed experimental system wasshown. On the other hand, using 60nm diameter fluorescent particles, flow analysis was performed. The technique is applicable for analyzing bio-fluid with bio-molecules tracer.