Anti-Platelet Aggregation and Plasma Fibrinolysis Accelerating Activities in Mushroom Extracts (Pleurotus ostreatus and Lentinus edodes)

Seven kinds of commercially obtained mushrooms were homogenized with 10 volume of distilled water or saline (wet/vol), and anti-platelet aggregation activity of each water-soluble fraction was compared. One-fortieth volume of Hiratake (Pleurotus ostreatus) and Shiitake (Lentinus edodes) extracts exhibited more than 50% of inhibition of primary and secondary aggregation of human PRP (220×106 platelet/ml) induced by ADP, collagen, or thrombin. They were dark yellow solutions, maintained more than 90% of anti-platelet aggregation activity after boiling at 100°C for 1h. Six volunteers, 18-37 years of age, were given lyophilized Hiratake extract (ASK-0P)with 500mg/kg body. After 1-3h, the platelet aggregation was decreased, whereas the fibrinolytic activity of EFA and t-PA were increased.